A DNA Extraction Kit for the Toughest Mining Samples

Interested in a trial kit? Email us at info@metagenom.com to get started! 

Uniquely engineered to overcome the obstacles of extracting DNA from low-biomass samples from demanding environments like coal, sand, rocks, acid mine drainage (AMD), and mine tailings, the Rox Low-Biomass Extreme Environments DNA Extraction Kit delivers industry-leading yields of high-quality DNA. Our proprietary methodology combines chemical and mechanical lysis, silica spin columns, and a unique chemical blend to produce exceptionally high yields of highly purified DNA—perfect for PCR, qPCR and high-throughput sequencing (HTS) applications. Rox is currently the only mine-water-optimized extraction kit on the market.

Sample Types

• Acid Mine Drainage (AMD)
• Mine Tailings
• Industrial Waters
• Highly Ionic Waters
• Low-Biomass Swabs
• CoalRock/stone surfaces

Performance Advantages

• Proven in Extreme Conditions: Successfully extracts DNA from sands, rocks, highly acidic (pH<2), and metal-laden environments such as AMD and tailings, ensuring reliable results in challenging mining samples
• More DNA: Delivers a higher yield of high-quality DNA than competing kits for many low-biomass samples, maximizing your sample’s potential
• Eliminates Extra Purification Steps: Effectively suppresses inhibitors such as heavy metals (e.g., arsenic, lead, and cadmium) and mineral contaminants (e.g., iron, sulfates, silicates, and sulfides), providing HTS- and PCR-ready DNA without additional cleanup
• Accurate Microbial Profile: Minimizes extraction bias, ensuring an accurate microbial community representation for reliable analysis of results

Ease of Use

• Save Time with Fast Extraction: Extract high-quality/yield DNA from 24 samples in just one hour, reducing sample processing time for time-sensitive experiments
• Safer and Simpler: Contains no organic solvents, such as phenol or chloroform, reducing health risks and allowing for more straightforward waste disposal
• Shelf-Stable for Convenience: can be stored at room temperature, eliminating the need for refrigeration and reducing storage costs

Validation

The Rox Low-Biomass Extreme Environments DNA Extraction Kit has been validated through successful DNA extraction from sand, rocks, coals, and diverse mining samples, followed by high-resolution 16S rRNA gene amplicon analysis.

Specifications

Catalogue Number: 18021_50

Sample Size: up to 250 mg

Elution Volume: >10 μl

Storage: Room temperature

Note: Not suitable for plant tissues or RNA extractions

Resources